The CRISPR/Cas9 system is widely used for various genome editing approaches in cultured cells and living organisms and was broadly explored for preclinical applications. This two component system is composed of the Cas9 endonuclease that acts in cooperation with a chimeric guide RNA (gRNA) mediating the sequence-specific binding to its complementary target protospacer sequence preceding a protospacer adjunct motif (PAM).

CRISPR/Cas9 PlatformCB provides a complete and powerful solution for CRISPR gene interference helping you to specifically suppress the expression of any gene. Our custom CRISPRi sgRNA vectors can be used on a variety of cells, including easy-to-transfect and difficult-to-transfect cells, covering the entire genome of humans, mice, and rats.

An emerging alternative solution is to express biosynthetic genes from the original producers in microbial hosts, notably bacteria and fungi. Prokaryotic and eukaryotic microbial cells, in combination with the advancement of recombinant DNA techniques, have been responsible for an explosion of bioproducts. Genetic engineered microbes can produce appreciable amounts of scarce products, thereby facilitating the synthesis of the target novel compound and potent derivatives.