Cell-based assays are important experimental tools in life science research and biomanufacturing. They are based on cell culture methods in which living cells are grown in vitro and used as a model system to assess the biochemistry and physiology of healthy and diseased cells. Cell culture assays provide a method to quantitatively analyze the presence, number, or functional activity of cells or tissues of interest. Both standardized and customized cell-based assays are widely used by scientists for numerous applications as cost-effective and reproducible research tools.